Optimized DNA sequence encoding Human GITR Ligand -GITRL (GLN50 - SER177 ) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human GITR Ligand(TNFSF18) is a homodimer protein consisting of 2x350 amino acid residue subunits,due to glycosylation migrates as an approximately as 46kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human GITR Ligand (GITRL) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human GITR Ligand can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The pathway between
necrosis factor (TNF)
protein (GITR) and GITR
ligand (GITRL) have been
shown to control the
function of regulatory T
GITRL is a type II
belonging to the TNF
superfamily, and cell
surface expression has been
observed on B cells,
macrophages, and dendritic
GITR Ligand provided by thrombopoietic cells inhibits nk cell antitumor activity
154 - 160.
a novel il-10–independent regulatory role for b cells in suppressing autoimmunity by maintenance of regulatory t cells via GITR Ligand J. Immunol.,
3188 - 3198.
th2 responses to helminth parasites can be therapeutically enhanced by, but are not dependent upon, gitr–GITR Ligand costimulation in vivo
1411 - 1420.
GITR Ligand–mediated local expansion of regulatory t cells and immune privilege of corneal allografts
Invest. Ophthalmol. Vis. Sci.,
6556 - 6565.
pseudoexpression of glucocorticoid-induced tnf-related (gitr) ligand upon coating of cancer cells by platelets impairs nk cell anti-tumor reactivity
Blood (ASH Annual Meeting Abstracts),