Optimized DNA sequence encoding Human Phospholipid Transfer Protein -PLTP (VAL18 - VAL493) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Phospholipid Transfer Protein(PTLP) is a protein consisting of 482 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human Phospholipid Transfer Protein (Lipid transfer protein II) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human PLTP can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Phospholipid Transfer Protein
Plasma phospholipid transfer
protein (PLTP) binds and
transfers a number of
vitamin E. It has been
implicated in several
including the production and
catabolism of very-low-
density and low-density
lipoproteins and the
remodeling of high-density
lipoproteins. Overall, PLTP
expression was found to
result in a higher incidence
and faster progression of
atherosclerosis in animal
models, and increased
expression of PLTP was most
frequently associated with
an increased risk of
cardiovascular disease in
Phospholipid Transfer Protein is expressed in cerebrovascular endothelial cells and involved in high density lipoprotein biogenesis and remodeling at the blood-brain barrier
J. Biol. Chem.,
4683 - 4698.
cathepsin g degradation of Phospholipid Transfer Protein (pltp) augments pulmonary inflammation
identification of a plasmodium falciparum Phospholipid Transfer Protein J. Biol. Chem.,
31971 - 31983.
liver-specific Phospholipid Transfer Protein deficiency reduces high-density lipoprotein and non–high-density lipoprotein production in mice
Arterioscler Thromb Vasc Biol,
2058 - 2064.
the role of Phospholipid Transfer Protein (pltp) in lipoprotein metabolism in the retina
Invest. Ophthalmol. Vis. Sci.,