Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human FGFBP1 Recombinant

FGFBP1

accession:  Q14512
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      500ug  $ 3500
Domain  : 
Gene  :  FGFBP1
Catalog no. :  RKQ14512

 

Source:

Optimized DNA sequence encoding Human Fibroblast growth factor binding protein- FGFBP1 (LYS24 - CYS234) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human FGF binding protein 1(FGF-BP1) is a protein consisting of 217 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological Activity
The activity was determined by the ability of FGFBP1 to bind recombinant human bFGF. The ED50 was calculated to be 10-50ng/ml
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human Fibroblast growth factor binding protein (FGF-BP1) is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Human FGFBP1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Fibroblast growth factor binding protein

Fibroblast growth factor- binding protein (FGF-BP) 1 is a secreted protein that can bind fibroblast growth factors (FGFs) 1 and 2. These FGFs are typically stored on heparan sulfate proteoglycans in the extracellular matrix in an inactive form, and it has been proposed that FGF-BP1 functions as a chaperone molecule that can mobilize locally stored FGF and present the growth factor to its tyrosine kinase receptor. FGF-BP1 is up- regulated in squamous cell, colon, and breast cancers and can act as an angiogenic switch during malignant progression of epithelial cells. Studies with FGF-BP1- negative cell lines showed that expression of FGF-BP1 increased tumor growth and angiogenesis in a nude mouse model. Complementary to that, depletion of endogenous FGF-BP1 from human squamous cell carcinoma and colorectal cancer cell lines reduced their tumor growth and angiogenesis.

Related Publications:
klf5 promotes breast cell survival partially through fibroblast growth factor-binding protein 1-perk-mediated dual specificity mkp-1 protein phosphorylation and stabilization
J. Biol. Chem., Jun 2009; 284: 16791 - 16798.
fibroblast growth factor-binding protein and n-deacetylase/n-sulfotransferase-1 expression in type ii cells is modulated by heparin and extracellular matrix
Am J Physiol Lung Cell Mol Physiol, Nov 2007; 293: L1314 - L1320.
effects on neurite outgrowth and cell survival of a secreted Fibroblast growth factor binding protein upregulated during spinal cord injury
Am J Physiol Regulatory Integrative Comp Physiol, Aug 2007; 293: R775 - R783.
expression of a fibroblast growth factor–binding protein during the development of adenocarcinoma of the pancreas and colon
Cancer Res., Jan 2006; 66: 1191 - 1198.
role of fibroblast growth factor-binding protein in the pathogenesis of hiv-associated hemolytic uremic syndrome
Am J Physiol Regulatory Integrative Comp Physiol, Jan 2006; 290: R105 - R113.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human FGFBP1 Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $