Optimized DNA sequence encoding Human Signal regulatory protein gamma - SIRP gamma (SIRP-β -2)(GLU29 - SER364 ) extracellular domain including a n IgG1 Fc C-terminal tag was expressed in HEK293 cells.
Recombinant Human SIRP gamma (SIRP-β -2) is a homodimer protein consisting of 2x368 amino acid residue subunits,due to glycosylation migrates as an approximately as 70kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human SIRP gamma (SIRPΒ2) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human SIRP gamma (SIRPB2) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
A third member of the SIRP
family, SIRPβ2, was recently
transcripts are variably
expressed in many human
tissues, including the
brain, lung, and placenta,
and are particularly
abundant in liver. The
predicted SIRPβ2 protein is
highly homologous to SIRPα
and SIRPβ1 in the
extracellular domain, but
lacks both cytoplasmic ITIMs
and the transmembrane lysine
required for association
endothelial cd47 interaction with sirp is required for human t-cell transendothelial migration under shear flow conditions in vitro
1280 - 1289.