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Human NKp30 Recombinant, Fc

NKp30

accession:  O14931
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  : 
Gene  :  NKp30
Catalog no. :  RFO14931

 

Source:

Optimized DNA sequence encoding Human Natural Killer Cell Receptor NKp30(LEU19 - GLY135) extracellular domain including a C-terminal Human IgG1 Fc tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human Natural Killer Cell Receptor (NCR3) Fc  is a homodimer protein consisting of 2x365 amino acid residue subunits,due to glycosylation migrates as an approximately as 90kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
Measured by its ability to bind immobilized recombinant Human B7-H6 in ELISA, the calculated ED50 is 10-50 ng/ml
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human NKp30 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Human NKp30 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

NKp30

The natural cytotoxicity receptors are a unique set of activating proteins expressed mainly on the surface of natural killer (NK) cells. The human natural cytotoxicity receptor family comprises the three type I membrane proteins NKp30, NKp44, and NKp46. Especially NKp30 is critical for the cytotoxicity of NK cells against different targets including tumor, virus- infected, and immature dendritic cells. NKp30 comprises an extracellular ligand binding domain, a transmembrane domain (TMD) that recruits the signaling adaptor protein CD3ζ, and a short cytoplasmic tail (6, 12). The N-terminal part of the ectodomain of NKp30 comprises an Ig-like fold (ligand-binding domain (LBD)) (29, 30), which is connected to the TMD by a flexible stalk domain.

Related Publications:
homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands
J. Biol. Chem., Jan 2014; 289: 765 - 777.
a soluble fragment of the tumor antigen bcl2-associated athanogene 6 (bag-6) is essential and sufficient for inhibition of NKp30 receptor-dependent cytotoxicity of natural killer cells
J. Biol. Chem., Nov 2013; 288: 34295 - 34303.
nk cells kill myeloma cells by increasing er stress and decreasing autophagy levels. nkg2d and NKp30 are involved in these processes
Blood, Nov 2013; 122: 3487.
hepatitis c virus–infected cells downregulate NKp30 and inhibit ex vivo nk cell functions
J. Immunol., Sep 2013; 191: 3308 - 3318.
downregulation of the activating NKp30 ligand b7-h6 by hdac inhibitors impairs tumor cell recognition by nk cells
Blood, Aug 2013; 122: 684 - 693.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human NKp30 Recombinant, Fc
product_icon_8.jpg Human NKp30 Recombinant
 
 
 
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