Optimized DNA sequence encoding Human Natural Killer Cell Receptor NKp44 (GLN22 - PRO190) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Natural Killer Cell Receptor (NCR2) is a protein consisting of 176 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human NKp44 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human NKp44 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The natural cytotoxicity
receptors are a unique set
of activating proteins
expressed mainly on the
surface of natural killer
(NK) cells. The human
receptor family comprises
the three type I membrane
proteins NKp30, NKp44, and
NKp46. NKp44 is expressed
only on activated NK cells
and was reported to be
involved in triggering NK
cells’ cytolytic activity
against both tumor and
identification of a cellular ligand for the natural cytotoxicity receptor NKp44 Blood,
2935 - 2942.
natural killer cells in hiv controller patients express an activated effector phenotype and do not up-regulate NKp44 on il-2 stimulation
11970 - 11975.
kaposi's sarcoma-associated herpesvirus orf54/dutpase downregulates a ligand for the nk activating receptor NKp44 J. Virol.,
8693 - 8704.
proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44 J. Immunol.,
5693 - 5702.
gut inflammation and indoleamine deoxygenase inhibit il-17 production and promote cytotoxic potential in NKp44+ mucosal nk cells during siv infection
3321 - 3330.