Optimized DNA sequence encoding mouse Kallikrein-11- hK11 (GLU19 - ASN250) preprotein including a C-terminal His tag was expressed in HEK293 cells.
Recombinant mouse hK11 (KLK11) is a protein consisting of 238 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse Kallikrein-11 (KLK11) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant hK11 (KLK11) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Mouse tissue kallikrein 11
(hK11) is a member of the
KLK family. hK11 is a
putative serine protease of
the human kallikrein gene
family. The gene encoding
hK11 was first cloned by
Yoshida et al. and was
named trypsin-like serine
protease. With the newly
established kallikrein gene
nomenclature, this gene is
now known as KLK11.With the
TLSP/hippostasin is now
known as hK11. This protein
is encoded by the KLK11
gene, which belongs to the
human kallikrein family
along with PSA (hK3) and
high level of anti-Kallikrein-11 ab in sjogren syndrome patients
Invest. Ophthalmol. Vis. Sci.,
validation and characterization of human kallikrein 11 as a serum marker for diagnosis of ovarian carcinoma
Clin. Cancer Res.,
4422 - 4428.
purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma
Clin. Cancer Res.,
742 - 750.
purification and characterization of human kallikrein 11, a candidate prostate cancer biomarker, from seminal plasma
AACR Meeting Abstracts,
the usefulness of serum human kallikrein 11 for discriminating between prostate cancer and benign prostatic hyperplasia
6543 - 6546.