Optimized DNA sequence encoding Human Kallikrein-4 - KLK4 (SER27 - SER254) preprotein including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Kallikrein-4 (KLK4) is a protein consisting of 234 amino acid residue subunits,due to glycosylation migrates as an approximately as 32kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to cleave a fluorogenic substrate Boc-VPR-AMC .The specific activity was measured to be 200 pmole/ug/min
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Kallikrein-4 (KLK-4) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Kallikrein-4 (KLK4) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Kallikrein 4 (KLK4) is a
member of the human KLK gene
family of serine proteases,
many of which are implicated
cancers. Like other KLKs,
such as KLK3/PSA and KLK2,
KLK4 gene expression is also
regulated by steroid
hormones in hormone-
dependent cancers, although
mechanisms are ill defined.
sat0006 microrna analysis of human lupus nephritis: evidence for modulation of kallikrein 4 by mir-422a
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effect of kallikrein 4 loss on enamel mineralization: comparison with mice lacking matrix metalloproteinase 20
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kallikrein 4 is a proliferative factor that is overexpressed in prostate cancer
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psa and kallikrein 4 expression in pc3 prostate cancer cells leads to a loss of cell adhesion molecules and an epithelial-mesenchymal transition (emt)
AACR Meeting Abstracts,
compartmentalized expression of kallikrein 4 (klk4/hk4) isoforms in prostate cancer: nuclear, cytoplasmic and secreted forms
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