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Human Kallikrein-4 Recombinant

KLK4

accession:  Q9Y5K2
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  KLK4
Catalog no. :  RKQ9Y5K2

 

Source:

Optimized DNA sequence encoding Human Kallikrein-4 - KLK4 (SER27 - SER254) preprotein including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human Kallikrein-4 (KLK4) is a protein consisting of 234 amino acid residue subunits,due to glycosylation migrates as an approximately as 32kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 

 
Biological Activity:
The activity was tested by the ability to cleave a fluorogenic substrate Boc-VPR-AMC .The specific activity was measured to be 200 pmole/ug/min
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Kallikrein-4 (KLK-4) is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Kallikrein-4 (KLK4) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Kallikrein-4

Kallikrein 4 (KLK4) is a member of the human KLK gene family of serine proteases, many of which are implicated in hormone-dependent cancers. Like other KLKs, such as KLK3/PSA and KLK2, KLK4 gene expression is also regulated by steroid hormones in hormone- dependent cancers, although the transcriptional mechanisms are ill defined.

Related Publications:
sat0006 microrna analysis of human lupus nephritis: evidence for modulation of kallikrein 4 by mir-422a
Ann Rheum Dis, Jun 2012; 71: 472 - 473.
effect of kallikrein 4 loss on enamel mineralization: comparison with mice lacking matrix metalloproteinase 20
J. Biol. Chem., May 2011; 286: 18149 - 18160.
kallikrein 4 is a proliferative factor that is overexpressed in prostate cancer
Cancer Res., Jun 2007; 67: 5221 - 5230.
psa and kallikrein 4 expression in pc3 prostate cancer cells leads to a loss of cell adhesion molecules and an epithelial-mesenchymal transition (emt)
AACR Meeting Abstracts, Apr 2006; 2006: 1027.
compartmentalized expression of kallikrein 4 (klk4/hk4) isoforms in prostate cancer: nuclear, cytoplasmic and secreted forms
Endocr. Relat. Cancer, Dec 2005; 12: 875 - 889.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human Kallikrein-4 Recombinant
 
 
 
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