Optimized DNA sequence encoding mature chain of human anterior gradient-2, AGR2 (ARG21 - LEU175 ) , including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human AGR2 is a protein consisting of 162 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human AGR-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant AGR2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Anterior Gradient Homolog 2
(AGR2) encodes a 17-kDa
protein that is highly
conserved in vertebrates.
AGR2 was first described in
Xenopus laevis where its
expression is responsible
for the development of a
glandular organ called the
cement gland .
AGR2 is also expressed by
secretory cells in the
normal murine intestine
(11). In humans, enhanced
AGR2 expression was first
described in breast cancer,
which was followed by
similar observations in most
including those derived from
the esophagus, pancreas,
lung, ovary, and prostate
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