Optimized DNA sequence encoding mature chain of Human B7-H4 (PHE29 - ALA258) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human B7H4 (VTCN1) is a protein consisting of 236 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The calculated ED50 is < 0.7 ug/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human B7-H4 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant B7-H4(B7S1) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
B7-H4 (B7x and B7S1) is a
member of the B7-CD28
superfamily with signature
IgV and IgC regions in the
extracellular domain, a
single transmembrane domain,
and an intracellular
domain.3 Although the mRNA
for B7-H4 is broadly
detectable in the majority
of normal tissues and cell
types, its cell surface
expression is relatively
rare.48 Expression of B7-H4
protein, however, can be
induced in vitro on
contrast to its limited
distribution in normal
tissues, constitutive cell
surface and cytoplasmic
expression of B7-H4 is found
on various cultured cancer
lines and freshly isolated
human carcinomas of breast,
lung, ovarian, and kidney
genetic variation in vtcn1 (b7-h4) is associated with course of disease in juvenile idiopathic arthritis
Ann Rheum Dis,
novel recombinant human B7-H4 antibodies overcome tumoral immune escape to potentiate t-cell antitumor responses
4820 - 4829.
host B7-H4 regulates antitumor t cell responses through inhibition of myeloid-derived suppressor cells in a 4t1 tumor transplantation model
6651 - 6661.
sat0180 b7-h4 expression of renal tubular epithelial cells in patients with lupus nephritis
Ann Rheum Dis,
enhanced t cell immunity by B7-H4 downregulation in nonsmall-cell lung cancer cell lines
Journal of International Medical Research,
497 - 506.