Optimized DNA sequence encoding extracellular domain of human Disintegrin and metalloproteinase 8 (ILE17 - LEU665) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human ADAM-8 (MS2) is a protein consisting of 656 amino acid residue subunits,due to glycosylation migrates as an approximately as 65kDa and 46kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human ADAM8 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant ADAM8 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
metalloprotease domain 8 is
expressed in immune,
neuronal, and bone
progenitor cells and is
thought to be involved in
ADAM8 behaves as an active
metalloprotease in vitro,
hydrolyzing myelin basic
protein and a variety of
peptide substrates based on
the cleavage sites of
growth factors, and
ADAM8 limits the development of allergic airway inflammation in mice
6434 - 6449.
cellular prion protein regulates its own α-cleavage through ADAM8 in skeletal muscle
J. Biol. Chem.,
16510 - 16520.
tumor necrosis factor- (tnf-) regulates shedding of tnf- receptor 1 by the metalloprotease-disintegrin ADAM8: evidence for a protease-regulated feedback loop in neuroprotection
12210 - 12218.
involvement of a disintegrin and a metalloproteinase 8 (ADAM8) in osteoclastogenesis and pathological bone destruction
Ann Rheum Dis,
427 - 434.
regulated expression of ADAM8 (a disintegrin and metalloprotease domain 8) in the mouse ovary: evidence for a regulatory role of luteinizing hormone, progesterone receptor, and epidermal growth factor-like growth factors
1038 - 1048.