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Human TIMP-2 Recombinant, Fc


accession:  P16035
   Size A     5ug   $ 160
   Size B      20ug  $480
   Size C      100ug  $ 1950
Domain  : 
Gene  :  TIMP-2
Catalog no. :  RFP16035



Optimized DNA sequence  encoding mature chain of human Metalloproteinase inhibitor 2 -TIMP-2(CYS27 - PRO220) including an N terminal IgG1 Fc- tag was expressed in HEK293 cells.

Molecular weight:

Recombinant human TIMP2 is a homodimer protein consisting of 430 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC
Biological activity:
The activity was tested by the ability of immobilized recombinant human MMP2 to bind recombinant human TIMP­-2 in ELISA, the calculated ED50 is 20ng/ml.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Human TIMP-2 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Recombinant TIMP2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Related Publications:
retinal mmp-12, mmp-13, timp-1, and TIMP-2 expression in murine experimental retinal detachment
Invest. Ophthalmol. Vis. Sci., Feb 2014; 10.1167/iovs.13-13374.
overexpression of endogenous TIMP-2 increases the proliferation of bewo choriocarcinoma cells through the mapk-signaling pathway
Reproductive Sciences, Oct 2013; 20: 1184 - 1192.
aging uniquely influences tissue inhibitor of metalloproteinase-2 (TIMP-2) expression in normal tendon
Br. J. Sports Med., Jun 2013; 47: e2.
enhanced immunoreactivity of TIMP-2 in the stromal compartment of tumor as a marker of favorable prognosis in ovarian cancer patients
Journal of Histochemistry & Cytochemistry, Jul 2012; 60: 491 - 501.
altered expressions of mmp-2, mmp-9, and TIMP-2 in placentas from women exposed to lead
Human and Experimental Toxicology, Jul 2012; 31: 662 - 670.

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