Optimized DNA sequence encoding mature chain of human Metalloproteinase inhibitor 2 -TIMP-2(CYS27 - PRO220) including an N terminal IgG1 Fc- tag was expressed in HEK293 cells.
Recombinant human TIMP2 is a homodimer protein consisting of 430 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human MMP2 to bind recombinant human TIMP-2 in ELISA, the calculated ED50 is 20ng/ml.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human TIMP-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant TIMP2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
retinal mmp-12, mmp-13, timp-1, and TIMP-2 expression in murine experimental retinal detachment
Invest. Ophthalmol. Vis. Sci.,
overexpression of endogenous TIMP-2 increases the proliferation of bewo choriocarcinoma cells through the mapk-signaling pathway
1184 - 1192.
aging uniquely influences tissue inhibitor of metalloproteinase-2 (TIMP-2) expression in normal tendon
Br. J. Sports Med.,
enhanced immunoreactivity of TIMP-2 in the stromal compartment of tumor as a marker of favorable prognosis in ovarian cancer patients
Journal of Histochemistry & Cytochemistry,
491 - 501.
altered expressions of mmp-2, mmp-9, and TIMP-2 in placentas from women exposed to lead
Human and Experimental Toxicology,
662 - 670.