Optimized DNA sequence encoding mature chain of Mouse Metalloproteinase inhibitor 1 -TIMP-1(CYS25-ARG205) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Mouse TIMP1 is a protein consisting of 190 amino acid residue subunits,due to glycosylation migrates as an approximately as 22kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of recombinant mouse TIMP2 to inhibit human MMP2 cleavage of a fluorogenic peptide substrate, the calculated ED50 is < 6nM.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse TIMP-1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant TIMP1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Tissue inhibitor of
metalloproteinases 1 (TIMP-
1) is a glycoprotein with a
relative molecular weight of
28,500 that is ubiquitously
expressed in numerous human
cells and tissues. TIMP-1
belongs to a group of four
endogenous inhibitors (TIMP-
1–TIMP-4) that control the
activity of matrix
metalloproteinases (MMP) and
other metalloproteinases .
In this respect, TIMP-1 is
an important regulator of
turnover. TIMP-1 was
discovered as a humoral
protein that promoted the
proliferation of human
erythroid progenitor .
a3.15 a novel role of mir-29a in inhibition of tab1-mediated TIMP-1 production in dermal fibroblasts: implication for ssc pathogenesis
Ann Rheum Dis,
A47 - A48.
retinal mmp-12, mmp-13, TIMP-1, and timp-2 expression in murine experimental retinal detachment
Invest. Ophthalmol. Vis. Sci.,
angiogenic capacity of m1- and m2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted prommp-9
4054 - 4067.
plasma tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) a powerful predictor of long term mortality in patients treated with cardiac resynchronization therapy
Eur. Heart J.,
toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors
Ann Rheum Dis,
1382 - 1389.