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Mouse TIMP-1 Recombinant

TIMP-1

accession:  P16035
   Size A     5ug   $ 160
   Size B      20ug  $550
   Size C      100ug  $ 1950
Domain  : 
Gene  :  TIMP-1
Catalog no. :  RKP16035

 

Source:

Optimized DNA sequence  encoding mature chain of Mouse Metalloproteinase inhibitor 1 -TIMP-1(CYS25-ARG205) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Mouse TIMP1 is a protein consisting of 190 amino acid residue subunits,due to glycosylation migrates as an approximately as 22kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by the ability of recombinant mouse TIMP2 to inhibit human MMP­2 cleavage of a fluorogenic peptide substrate, the calculated ED50 is < 6nM.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Mouse TIMP-1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant TIMP1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TIMP-1

Tissue inhibitor of metalloproteinases 1 (TIMP- 1) is a glycoprotein with a relative molecular weight of 28,500 that is ubiquitously expressed in numerous human cells and tissues. TIMP-1 belongs to a group of four endogenous inhibitors (TIMP- 1–TIMP-4) that control the activity of matrix metalloproteinases (MMP) and other metalloproteinases . In this respect, TIMP-1 is an important regulator of extracellular matrix turnover. TIMP-1 was discovered as a humoral protein that promoted the proliferation of human erythroid progenitor .

Related Publications:
a3.15 a novel role of mir-29a in inhibition of tab1-mediated TIMP-1 production in dermal fibroblasts: implication for ssc pathogenesis
Ann Rheum Dis, Mar 2014; 73: A47 - A48.
retinal mmp-12, mmp-13, TIMP-1, and timp-2 expression in murine experimental retinal detachment
Invest. Ophthalmol. Vis. Sci., Feb 2014; 10.1167/iovs.13-13374.
angiogenic capacity of m1- and m2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted prommp-9
Blood, Dec 2013; 122: 4054 - 4067.
plasma tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) a powerful predictor of long term mortality in patients treated with cardiac resynchronization therapy
Eur. Heart J., Aug 2013; 34: P3186.
toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors
Ann Rheum Dis, Aug 2013; 72: 1382 - 1389.




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Antibodies:
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Related Products:
product_icon_8.jpg Human TIMP-1 Recombinant
product_icon_8.jpg Mouse TIMP-1 Recombinant
 
 
 
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