Optimized DNA sequence encoding mature chain of human Angiotensinogen -AGT (Asp34 - Ala485) including an N-terminal His tag was expressed in HEK293 cells.
Recombinant human AGT is a protein consisting of 458 amino acid residue subunits,due to glycosylation migrates as an approximately 60kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Angiotensinogen (AGT) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Angiotensinogen can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
synthesized primarily in
cells of the proximal tubule
and is secreted from the
apical surface into the
lumen, where it is converted
first to angiotensin (Ang) I
and then Ang II by tubular
renin and angiotensin-
converting enzyme (ACE),
association between urinary Angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia
Journal of Renin-Angiotensin-Aldosterone System,
dynamic ccaat/enhancer binding protein–associated changes of dna methylation in the Angiotensinogen gene
281 - 288.
rock/nfb axis-dependent augmentation of Angiotensinogen by angiotensin ii in primary-cultured preglomerular vascular smooth muscle cells
Am J Physiol Renal Physiol,
human Angiotensinogen +11525 c/a polymorphism modulates its gene expression through microrna binding
901 - 906.
epigenetic control of Angiotensinogen and cyp11b2 gene expression in the hypertrophic hearts
Eur. Heart J.,