Cart contains    items

Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human Tissue factor pathway inhibitor Recombinant


accession:  P10646
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  TFPI
Catalog no. :  RKP10646



Optimized DNA sequence  encoding mature chain of human Tissue factor pathway inhibitor -TFPI (ASP29 - LYS282) including a C-terminal His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant human TFPI is a protein consisting of 261 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa band protein on reduced SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC
Biological activity:
The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE- Nval-WRKDnp-NH2, with a calculated ED50 < 3.5nM
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Human Tissue factor pathway inhibitor is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Recombinant Tissue factor pathway inhibitor  can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Tissue factor pathway inhibitor

TF-induced coagulation is regulated by a specific inhibitor termed tissue factor pathway inhibitor (TFPI). The mechanism of inhibition by TFPI involves its binding first to factor Xa (FXa) and then to the FVIIa TF complex. TFPI is a multivalent serine protease inhibitor with 3 independently folded Kunitz- type proteinase inhibitor domains and a highly basic, positively charged carboxyl terminus known to bind heparin. The first Kunitz domain (K1) binds TF/VIIa, and the second Kunitz domain binds factor Xa. It is the formation of this quaternary TF-VIIa-TFPI-Xa complex that dampens ongoing coagulatio

Related Publications:
small peptides blocking inhibition of factor xa and tissue factor-factor viia by Tissue factor pathway inhibitor (tfpi)
J. Biol. Chem., Jan 2014; 289: 1732 - 1741.
protein s is a cofactor for platelet and endothelial tissue factor pathway inhibitor-α but not for cell surface–associated tissue factor pathway inhibitor
Arterioscler Thromb Vasc Biol, Jan 2014; 34: 169 - 176.
translation of human tissue factor pathway inhibitor-β mrna is controlled by alternative splicing within the 5' untranslated region
Arterioscler Thromb Vasc Biol, Jan 2014; 34: 187 - 195.
crystal structure of Tissue factor pathway inhibitor (tfpi) kunitz domain 1 and 2 in complex with an inhibitory cyclic peptide,
Blood, Nov 2013; 122: 453.
the potential to enhance hemostasis by inhibiting tissue factor pathway inhibitor
Blood, Nov 2013; 122: SCI-9.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Tissue factor pathway inhibitor 2 Recombinant
product_icon_8.jpg Human Tissue factor pathway inhibitor 2 Recombinant
product_icon_8.jpg Mouse Tissue factor pathway inhibitor Recombinant
product_icon_8.jpg Human Tissue factor pathway inhibitor Recombinant
Item name Size Quantity Price(USD) Remove
Cart Total $