Mouse NEPH1 Recombinant

Category:

$160.00$1,950.00

SKU: RKQ80W68 Tags: , , ,

Description

Accession
Q80W68
Source
Optimized DNA sequence encoding extracellular domain of NEPH1 (LEU48 - LEU525 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant mouse NEPH1 is a protein consisting of 485 amino acid residue subunits,due to glycosylation migrates as an approximately 70kDa band protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological activity
The activity was tested by the ability of immobilized recombinant mouse NEPH1 to bind mouse MS-1 endothelial cells, after 90 min 70% of cells will adhere.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse NEPH1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Recombinant NEPH1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading