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Human NEPH1 Recombinant

NEPH1

accession:  Q96J84
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  : 
Gene  :  NEPH1
Catalog no. :  RKQ96J84

 

Source:

Optimized DNA sequence  encoding extracellular domain of NEPH1 (GLN17 - ALA499 ) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant human NEPH1 is a protein consisting of 491 amino acid residue subunits,due to glycosylation migrates as an approximately 70kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by the ability of immobilized recombinant human NEPH1 to bind mouse MS-1 endothelial cells, after 90 min 75% of cells will adhere.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human NEPH1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant NEPH1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

NEPH1

NEPH1, a molecule recently identified in mice by a retrovirus-mediated mutagenesis screen, contains five extracellular immunoglobulin-like domains and is structurally related to nephrin (7). NEPH1 is abundantly expressed in the kidney, and disruption of the NEPH1 gene in mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death. Nephrin and Neph1 are structurally similar type I transmembrane proteins of the immunoglobulin superfamily. They directly interact in the plane of the plasma membrane via their cytoplasmic domains (4, 12, 25). In addition, the Nephrin extracellular domain interacts with itself and with Neph1 via a trans- interaction across the mature podocyte intercellular junction; for this reason, it has been presumed that these proteins mediate cell-cell adhesive interactions at this site

Related Publications:
slit diaphragm protein NEPH1 and it's signaling: a novel therapeutic target for protection of podocytes against glomerular injury
J. Biol. Chem., Feb 2014; 10.1074/jbc.M113.505743.
solution structure analysis of cytoplasmic domain of podocyte protein NEPH1 using small/wide angle x-ray scattering (swaxs)
J. Biol. Chem., Mar 2012; 287: 9441 - 9453.
motor protein myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein NEPH1 to the podocyte membrane
Mol. Cell. Biol., May 2011; 31: 2134 - 2150.
recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the drosophila retinotopic map
Development, Oct 2010; 137: 3303 - 3313.
NEPH1 regulates steady-state surface expression of slo1 ca2+-activated k+ channels: different effects in embryonic neurons and podocytes
Am J Physiol Cell Physiol, Dec 2009; 297: C1379 - C1388.




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Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Mouse NEPH1 Recombinant
product_icon_8.jpg Human NEPH1 Recombinant
 
 
 
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