Optimized DNA sequence encoding fibronectin (LYS2175 - GLU2386) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human Fibronectin is a protein consisting of 220 amino acid residue subunits,due to glycosylation migrates as an approximately 28kDa bands protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Fibronectin is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Fibronectin can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
FN is a major component of
the provisional ECM produced
angiogenesis. β1 integrin
and FN fibrillogenesis are
crucial for endothelial
tubulogenesis and branching,
mural cell apposition, and
regulation of lumen diameter
definition of the native and denatured type ii collagen binding site for Fibronectin using a recombinant collagen system
J. Biol. Chem.,
4941 - 4951.
the eda-containing cellular Fibronectin induces epithelial–mesenchymal transition in lung cancer cells through integrin α9β1-mediated activation of pi3-k/akt and erk1/2
184 - 191.
the structure of irisin reveals a novel intersubunit β-sheet Fibronectin type iii (fniii) dimer: implications for receptor activation
J. Biol. Chem.,
33738 - 33744.
contribution of platelet integrins and shear to the unfolding of Fibronectin Blood,
calcitonin-induced nf-b activation up-regulates Fibronectin expression in mg63 osteosarcoma cells
4901 - 4906.