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Human R-Spondin1 Recombinant, Biotinylated

RSPO1

accession:  Q2MKA7
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  : 
Gene  :  RSPO1
Catalog no. :  RBQ2MKA7

 

Source:

Optimized DNA sequence  encoding peptidase domain of Human R-Spondin1(SER21-ALA263 ) including a C-terminal His tag was expressed in HEK293 cells. Recombinant R-spondin1 was further labled by Biotin for detection by avidin conjugated secondary probes.


 
Molecular weight:

Recombinant human R-Spondin-1 is a protein consisting of 433 amino acid residue subunits,due to glycosylation migrates as an approximately 53kDa protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Applications:

ELISA,WB

Biotinylated recombinant R-spondin1 was designed to be used in single step detection in ELISA ,WB

 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human R-spondin1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant R-spondin1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

R-Spondin1

The R-Spondin (RSpo) family of secreted proteins is implicated in the amplification of canonical Wnt/β-catenin signaling pathway both in vitro and in vivo. All four RSpo family members contain an N- terminal signal peptide, two furin-like domains, followed by a thrombospondin type1 domain, and a C-terminal region rich with positively charged amino acids. The Wnt/β-catenin signaling plays a pivotal role in both embryonic development and homeostatic self-renewal of adult tissues, including gastrointestinal (GI) and oral mucosa. Human RSPO1 was first identified by its ability to stimulate the growth of intestinal epithelium in transgenic mice. The therapeutic potential of the RSPO1 protein was also demonstrated in mouse models of cancer-therapy-induced mucositis and experimental colitis

Related Publications:
the wnt agonist R-Spondin1 regulates systemic graft-versus-host disease by protecting intestinal stem cells
J. Exp. Med., Feb 2011; 208: 285 - 294.
R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical wnt/β-catenin pathway
PNAS, Feb 2009; 106: 2331 - 2336.
use of R-Spondin1, an intestinotrophic mitogen, in the treatment of murine graft-versus-host disease
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3520.
protection of the intestinal epithelium from conditioning with R-Spondin1 ameliorates graft-versus-host disease
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 66.
crosstalk between ahr and wnt signaling through R-Spondin1 impairs tissue regeneration in zebrafish
FASEB J, Aug 2008; 22: 3087 - 3096.




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Antibodies:
Purified Polyclonal
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