Optimized DNA sequence encoding extracellular domain of Human Programmed cell death protein 1 PD-1 (PRO21 - GLN167) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human PD-1 (PDCD1) is a protein consisting of 150 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Human PD-1 to bind recombinant human PD-L1 within a linear range of 100-1000ng/ml in ELISA.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human PD-1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in sterile PBS pH 7.4 to a concentration not less than 0.2 mg/mL. Solubilize for 1hr at room temp with gentle mixing.
Recombinant PD-1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.It is recommended to add 0.1% HSA or BSA for long term storage.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The programmed cell death-1
(PD-1) is an inhibitory
surface receptor expressed
by T cells, B cells, natural
killer T cells, monocytes,
and dendritic cells (DC),
but not by resting T cells.
PD-1 binds two ligands,
programmed cell death ligand
1 (PD-L1) and PD-L2 called
B7-H1 and B7-DC,
respectively . Tumors can
use the PD-1 inhibitory
pathway to silence the
differential impact of PD-1 and/or interleukin-10 blockade on hiv-1-specific cd4 t cell and antigen-presenting cell functions
2508 - 2518.
PD-1 and tim-3 regulate the expansion of tumor antigen–specific cd8+ t cells induced by melanoma vaccines
1045 - 1055.
negative regulation by pd-l1 during drug-specific priming of il-22–secreting t cells and the influence of PD-1 on effector t cell function
PD-1 blockage delays murine squamous cell carcinoma development
424 - 431.
highly pathological influenza a virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific cd8+ t cells
1636 - 1651.