Optimized DNA sequence encoding extracellular domain of Mouse Proprotein convertase subtilisin/kexin type 9 (PCSK9) (GLN35 - GLN694) including an N-terminal His tag was expressed in HEK293 cells.
Recombinant Mouse PCSK9 is a protein consisting of 670 amino acid residue subunits,due to glycosylation migrates as an approximately 20kDa & 60kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Mouse PCSK9 to bind recombinant human LDLR within a linear range of 10-100ng/ml in ELISA.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse PSCK9 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant PCSK9 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
LDLR also binds proprotein
type-9 (PCSK9), a secreted
member of the mammalian
proprotein convertase family
of serine endoproteases .
Bound PCSK9 disrupts the
normal endocytic recycling
itinerary of the LDLR and
directs the receptor for
degradation in endolysosomal
compartments. PCSK9 binds in
a Ca2+-dependent manner to
the first of three Ca2+-
binding EGF-like repeats
(EGF-A) in the EGF precursor
homology domain of LDLR and
binding affinity at acidic
internalized PCSK9 dissociates from recycling ldl receptors in PCSK9-resistant sv-589 fibroblasts
J. Lipid Res.,
266 - 275.
identification of a small peptide that inhibits PCSK9 protein binding to the low density lipoprotein receptor
J. Biol. Chem.,
942 - 955.
loss of plasma proprotein convertase subtilisin/kexin 9 (PCSK9) after lipoprotein apheresis
1290 - 1295.
characterization of the role of egf-a of low density lipoprotein receptor in PCSK9 binding
J. Lipid Res.,
3345 - 3357.
role of PCSK9 and idol in the pathogenesis of acquired ldl receptor deficiency and hypercholesterolemia in nephrotic syndrome
Nephrol. Dial. Transplant.,