Optimized DNA sequence encoding extracellular domain of Human Angiopoietin-2 (TYR19-PHE496) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Angiopoietin-2 was further labled by Biotin for detection by avidin conjugated secondary probes.
Recombinant Angpt2 is a protein consisting of 490 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Human Tie2 to bind biotinylated recombinant human Angiopoietin-2 in ELISA
Biotinylated recombinant ANG-2 was designed to be used in single step detection in ELISA ,WB
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Angiopoietin-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant Angiopoietin-2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Angiopoietin-2 (Ang2)2 is a
70-kDa secreted ligand whose
increased expression has
been implicated in a range
of diseases, including
cancer, sepsis, and acute
syndrome. The primary
receptor for Ang2 is the
kinase Tie2 (3) that is
expressed mainly on vascular
endothelial cells and
myeloid cells (1, 4). Ang2
plays an important role in
vascular remodeling during
development, but in adult
tissues, Ang2 concentrations
are usually low. An increase
in Ang2 levels in disease
allows the molecule to
compete for binding to a
common interface on Tie2
with the related agonist
Angiopoietin-2–induced arterial stiffness in ckd
J. Am. Soc. Nephrol.,
endothelial cell-derived Angiopoietin-2 controls liver regeneration as a spatiotemporal rheostat
416 - 419.
Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (pi3k)/akt/endothelial nitric oxide synthase (enos) and syndecan-4/syntenin pathways
J. Biol. Chem.,
510 - 519.
thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through Angiopoietin-2 upregulation
1331 - 1344.
circulating Angiopoietin-2, its soluble receptor tie-2, and mortality in the general population
Eur J Heart Fail,
1327 - 1334.