/, Biotinylated proteins, Cytokines/Human VEGF 121AA Recombinant (CHO Cell), Biotinylated

Human VEGF 121AA Recombinant (CHO Cell), Biotinylated

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$340.00$1,950.00

SKU: RKB15692 Tags: , , , ,

Description

Accession
P15692
Source
Optimized DNA sequence encoding Human VEGF mature chain fused to N terminal AVI polyhis tag was expressed in CHO Cells,was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight
Native human VEGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 17kDa. Recombinant Vascular endothelial growth factor is a monomer protein , and migrates as an approximately 45 kDa protein under non-reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Applications
ELISA,WB Biotinylated VEGF-121 was designed to be used in single step detection in ELISA ,WB
Protein Sequence
MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NPCGPCSERR KHLFVQDPQT CKCSCKNTDS RCKARQLELN ERTCRCDKPR R
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
RecombinantVEGF was lyophilized from.2 μm filteredmM PB solution, pH7.0 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
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