Optimized DNA sequence encoding cytoplasmic domain of human CD20 including a N-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD20 is a monomer protein consisting of 105 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa protein on SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD20 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD20, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD20 is a signature B-cell
strongly expressed on the
surface of mature normal as
well as malignant B cells.
It is also expressed, but at
lower levels and with larger
variance, on more immature B
cells and their malignant
counterparts found in B-cell
precursor (BCP) acute
reduced t-dependent humoral immunity in CD20-deficient mice
3112 - 3118.
recombinant human il-15 trans-presentation by b leukemic cells from chronic lymphocytic leukemia induces autologous nk cell proliferation leading to improved anti-CD20 immunotherapy
combination treatment with anti-CD20 and oral anti-cd3 prevents and reverses autoimmune diabetes
2849 - 2858.
salmonella engineered to express CD20-targeting antibodies and a drug-converting enzyme can eradicate human lymphomas
705 - 714.
preclinical activity of the type ii CD20 antibody ga101 (obinutuzumab) compared with rituximab and ofatumumab in vitro and in xenograft models
Mol. Cancer Ther.,