//Mouse CD40 (TNFRSF5) Recombinant

Mouse CD40 (TNFRSF5) Recombinant

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$160.00$1,650.00

SKU: RKP27512 Tags: , , , , ,

Description

Accession
P27512
Source
Optimized DNA sequence encoding extracellular domain of Mouse CD40 including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant CD40 is a protein consisting of 180 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein on SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant mouse CD40 to bind Human CD40 Ligand with a linear range of 10-500 ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD40 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant CD40 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
B2RUR8
Interactor
O08863
Interactor
P39429
Interactor
Q60803
Biological Process
Molecular function

Methods

Surface Ags on U266 cells treated by IFN-γ or BCGV.

  • Surface HLA-DR, CD40, CD80, CD86 and CD54 on tumor cells were examined by flow cytometry.
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