Biotinylated Human Serum Albumin Protein Recombinant
Category: Biotinylated proteins$155.00 – $515.00
Description
Accession
P02768
Source
DNA sequence encoding Human Serum Albumin including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Serum Albumin is a protein consisting of amino acid residues [Asp25-Leu609] ,The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Serum Albumin was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O41931
Interactor
O41970
Interactor
Interactor
P88993
Interactor
P89884
Methods
Biotinylated Human Serum Albumin Protein Tris-Bis PAGE
- Biotinylated Human Serum Albumin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Serum Albumin Protein SEC-HPLC
- The purity of Biotinylated Human Serum Albumin is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Serum Albumin Protein SPR Data
- Biotinylated Human Serum Albumin, His-Avi Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 1.67 μM as determined in SPR assay (Biacore T200).
