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Human Leptin receptor Fc tag Recombinant (LEPR)

LEP-R

accession:  P48357
   Size A     50ug   $ 160
   Size B      200ug  $480
   Size C      1000ug  $ 1950
Domain  : 
Gene  :  LEPR
Catalog no. :  RKP483571

Source:
Optimized DNA sequence  encoding extracellular domain of human leptin receptor  including a C-terminal Human IgG1 Fc tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant human leptin receptor is a homodimer protein consisting of two 1070 amino acid residue subunit chains,  due to glycosylation migrates as an approximately 160kDa protein on SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Measured by its ability to bind immobilized recombinant human Leptin (1ug/ml) at a linear concentration range of 0.01-4 ug/ml .

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human leptin receptor is supplied as lyophilized 0.2 μm filtered PBS solution,  pH7.2 .

              
Storage:
Recombinant human Leptin receptor, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Leptin receptor

Leptin exerts its activity through specific membrane receptor, the obesity or leptin receptor (LEP-R), which is assigned to class I cytokine receptor family. Six splice variants of LEP-R have been identified up to now; a long isoform, four short isoforms discriminated by the different lengths of intracellular domain and the secreted isoform, which modulates blood leptin . According to the current knowledge, leptin-signaling pathway is mainly transduced by janus kinases/signal transducers and activators of transcription, mitogen- activated protein kinase and phosphatidylinositol 3- Kinase (PI3K)-signaling pathways. PI3K/AKT pathway may be set off through the activation of receptor tyrosine kinases by certain growth and survival factors (including Ob), through the activation of non-receptor tyrosine kinases or through Ras- signaling pathway activation . Activated PI3K phosphorylates phosphatidylinositol-3, 4- biphosphate and phosphatidylinositol-3, 4, 5-triphosphate. Phosphatidylinositol-3, 4- biphosphate and phosphatidylinositol-3, 4, 5-triphosphate activate a downstream target of PI3K namely AKT kinase that subsequently activate other targets including glycogen synthase kinase 3, FoxO1 and p27kip1.

Related Publications:
acyclic retinoid inhibits diethylnitrosamine-induced liver tumorigenesis in obese and diabetic c57blks/j- +LEPRdb/+LEPRdb mice
Cancer Prevention Research, Jan 2011; 4: 128 - 136.
the triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic-acid methyl ester has potent anti-diabetic effects in diet-induced diabetic mice and LEPRdb/db mice
J. Biol. Chem., Dec 2010; 285: 40581 - 40592.
leptin receptor (LEPR) is a negative modulator of bone mechanosensitivity and genetic variations in LEPR may contribute to the differential osteogenic response to mechanical stimulation in the c57bl/6j and c3h/hej pair of mouse strains
J. Biol. Chem., Nov 2010; 285: 37607 - 37618.
genome-wide association study identifies polymorphisms in LEPR as determinants of plasma soluble leptin receptor levels
Hum. Mol. Genet., May 2010; 19: 1846 - 1855.
no association between polymorphisms in lep, LEPR, adipoq, adipor1, or adipor2 and postmenopausal breast cancer risk
Cancer Epidemiol. Biomarkers Prev., Sep 2009; 18: 2553 - 2557.




by class:
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by species:
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mouse
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Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Leptin receptor Recombinant (LEPR)
product_icon_8.jpg Human Leptin receptor Fc tag Recombinant (LEPR)
 
 
 
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