//Human IL-21R (CD360) Recombinant

Human IL-21R (CD360) Recombinant

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$160.00$1,950.00

SKU: RKQ9HBE5 Tags: , , , , , ,

Description

Accession
Q9HBE5
Source
Optimized DNA sequence encoding extracellular domain of human IL-21R receptor including a C terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant IL-21R is a monomer protein consisting of 228 amino acid residue subunits, due to glycosylation migrates as an approximately45kD protein under reducing SDS-PAGE conditions.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Measured by its ability to inhibit IL21 induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells .
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-21receptor is supplied as lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Storage
Recombinant IL-21R, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function

Methods

IL-2/IL-21 and upregulation of IL-21R expression replace CD4+ T cell help of CD8+ T cell expansion in vitro.

  • On the left, histogram plots for 1 donor is shown and, on the right, IL-21R expression on day 4 is displayed for 5 donors.

Cytokine protein analysis

  • MFBI Th1/Th2 Flow Cytomix Multiplex kits (eBioscience) were used to measure the concentration of cytokines in the serum two hours after s.c. treatment with soluble peptide, while IL-21 and IL-10 Flow Cytomix simplex kits (both eBioscience) were used to assay cell culture supernatant.
  • Fluorescence intensity was measured on a FACS Calibur flow cytometer and data were analysed using FlowCytomix Pro software (eBioscience).
  • Conventional sandwich ELISA were performed to quantify cytokine concentration in cell culture supernatant (harvested at 24 hours after re-stimulation for IL-2, at 72 hours for IFN-γ and IL-10) using matched antibody pairs (all ).
  • IL-2 coating, JES6-1A12 (2μg ml−1), biotinylated, JES6-5H4 (0.5μg ml−1).
  • IFN-γ: coating R4-6A2 (2μg ml−1), biotinylated, XMG1.2 (0.5μg ml−1).
  • IL-10: coating JES5-2A5 (2μg ml−1), biotinylated SXC-1 (0.5μg ml−1).
  • Optical change was measured witha SpectraMax 190 microplate reader ; cytokine concentration was calculated using Microplate Manager software .
  • Intracellular cytokine staining of splenocytes was performed after a 3 hour stimulation with…
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