Human IL-1 receptor type II Recombinant (CD121b)
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
P27930
Source
Optimized DNA sequence encoding Human extracellular domain of human IL-1 receptor type II including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant IL-R2 (CD121b) is a protein consisting of342 amino acid residue subunits, due to glycosylation migrates as an approximately50-60 kDa protein on SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Recombinant Human IL-1 receptor type 2 activity was measured by its ability to inhibit IL-1b dependent proliferation in mouse helper T cells. The ED50 for this effect is typically 5 μg/mL in the presence of 40 pg/mL of human IL-1b.
Presentation
Recombinant Human IL-1 receptor II (CD121b) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant Human IL1R2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Determination of hIL-1RI, hIL-1RII, and hIL-1RAcP binding
- A recombinant version of wild type human IL-1β carrying a C-terminal hexahistidine tag and additional cysteine residue (hIL-1β-His-C) was site specifically biotinylated at its free cysteine residue using an EZ-link maleimide-PEG11-biotinylation kit .
- Biotinylated hIL-1β-His-C bound with the same affinity as wild-type human hIL-1β to immobilized recombinant human IL-1I and IL-II Fc chimeras , as shown byELISA using polyclonal goat antihuman IL-1β IgG for detection (data not shown).
- For determination of binding affinities, serial dilutions of nonlabeled wild-type human IL-1β or hIL-1b(145K) were premixed with a constant amount of 20 ng/ml (~1 nmol/l) of biotinylated hIL-1β-His-C and then transferred to ELISA plates ( eBioscience, Vienna, Austria) that had been coated with 1 µg/ml of human IL-1I or human IL-II Fc chimeras .
- Bound biotinylated hIL-1β-His-C was detected with horseradish peroxidase–conjugated streptavidin.
- Binding curves were fitted by four-parameter logistic equations using GraphPad Prism (GraphPad Software, La Jolla, CA), and inhibition data were reciprocally transformed…