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Human TNFR2 Recombinant (CD120b)


accession:  P20333
   Size A       $
   Size B      50ug  $350
   Size C      500ug  $ 1950
Domain  :  Ig-like C2
Gene  :  TNFR2
Catalog no. :  RKP20333

Optimized DNA sequence  encoding  extracellular domain of Human TNF receptor type 2 (TNFR2)  including a C-terminal 6His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant human TNF receptor 2 (CD120b) is a protein consisting of 248 amino acid residue subunits, due to glycosylation migrates as an approximately 45kDa protein on SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC

Biological Activity:

Recombinant Human TNF receptor 2 (CD120b) activity was determined by the ability to inhibit the TNFα mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

The ED50 for this effect is typically 0.2-0.8 μg/mL in the presence of 0.15 ng/mL recombinant Human TNFα .



Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant human TNFR2 (TNFRSF1B) is supplied as a 0.2 μm filtered PBS solution, pH7.2.

Recombinant Human TNF receptor 2 (CD120b), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



The effects of TNFα are mediated by two distinct cell surface receptors, p55 TNF-R1 and p75 TNF-R2, that are expressed on almost all nucleated cells. Both receptors lack recognizable enzymatic domains, and their ability to transduce signals is dependent on the interaction with proteins associating with their cytoplasmic tails. Like other members of the tumor necrosis factor (TNF) receptor family, p55 TNF receptor 1 (TNF-R1) lacks intrinsic signaling capacity and transduces signals by recruiting associating molecules. The TNF-R1 associated death domain protein interacts with the p55 TNF-R1 cytoplasmic domain and recruits the Fas- associated death domain protein.

Related Publications:
apoptosis and expression of antiviral response genes during ocular hsv-1 infection in tnfr1 or TNFR2 knockout mice
Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 6158.
association of tnfα, tnfr1, and TNFR2 polymorphisms with sperm concentration and motility
J Androl, Jan 2012; 33: 74 - 80.
tnf-induced ip3r1 expression through tnfr1/pc-plc/pkc and TNFR2 signalling pathways in human mesangial cell
Nephrol. Dial. Transplant., Jan 2011; 26: 75 - 83.
solution of the structure of the tnf-TNFR2 complex
Sci. Signal., Nov 2010; 3: ra83.
expression of costimulatory TNFR2 induces resistance of cd4+foxp3– conventional t cells to suppression by cd4+foxp3+ regulatory t cells
J. Immunol., Jul 2010; 185: 174 - 182.

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