Optimized DNA sequence encoding extracellular domain of Mouse TNF receptor type 2 (TNFR2) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant Mouse TNF receptor 2 (CD120b) is a protein consisting of 248 amino acid residue subunits, due to glycosylation migrates as an approximately 45kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant Mouse TNF receptor 2 (CD120b) activity was determined by the ability to inhibit the TNFα mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 1-3 μg/mL in the presence of 0.15 ng/mL recombinant mouse TNFα .
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse TNFR2 (TNFRSF1B) is supplied as a 0.2 μm filtered PBS solution, pH7.2.
Recombinant Mouse TNF receptor 2 (CD120b), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The effects of TNFα are
mediated by two distinct
cell surface receptors, p55
TNF-R1 and p75 TNF-R2, that
are expressed on almost all
nucleated cells. Both
receptors lack recognizable
enzymatic domains, and their
ability to transduce signals
is dependent on the
interaction with proteins
associating with their
Like other members of the
tumor necrosis factor (TNF)
receptor family, p55 TNF
receptor 1 (TNF-R1) lacks
intrinsic signaling capacity
and transduces signals by
molecules. The TNF-R1
associated death domain
protein interacts with the
p55 TNF-R1 cytoplasmic
domain and recruits the Fas-
associated death domain
apoptosis and expression of antiviral response genes during ocular hsv-1 infection in tnfr1 or TNFR2 knockout mice
Invest. Ophthalmol. Vis. Sci.,
association of tnfα, tnfr1, and TNFR2 polymorphisms with sperm concentration and motility
74 - 80.
tnf-induced ip3r1 expression through tnfr1/pc-plc/pkc and TNFR2 signalling pathways in human mesangial cell
Nephrol. Dial. Transplant.,
75 - 83.
solution of the structure of the tnf-TNFR2 complex
expression of costimulatory TNFR2 induces resistance of cd4+foxp3– conventional t cells to suppression by cd4+foxp3+ regulatory t cells
174 - 182.