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Human CD117 Recombinant (SCFR)

SCFR

accession:  P10721
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  :  Ig-like C2
Gene  :  SCFR
Catalog no. :  RKP15509

Source:
Optimized DNA sequence  encoding  extracellular domain of human SCF receptor (CD117) isoform 2 including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant C-Kit receptor (CD117) is a monomer protein consisting of 502 amino acid residue subunits, due to glycosylation migrates as an approximately 80kDa protein on SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
 
Biological Activity:

Recombinant CD117 activity was measured by its binding ability in a functional ELISA. Immobilized human KIT at 20 μg/ml can bind biotinylated mouse KITL with a linear ranger 1 - 6 ng/ml

 

Endotoxin:

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human c-kit receptor is supplied as a 0.2 μm filtered PBS solution, pH7.2.

              
Storage:
Recombinant human CD117, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

C-KIT receptor

The c-Kit receptor consists of an extracellular portion with five immunoglobulin- like regions and an intracellular tyrosine kinase domain. The first three immunoglobulin-like domains form a binding site for its ligand, stem cell factor (SCF), the fourth is involved in c-Kit receptor dimerization, and the function of the fifth is, as yet, unknown. Binding of SCF induces homodimerization of the c- Kit receptor, followed by an increase in receptor autophosphorylation activity and recruitment of the c-Kit signaling complex.

Related Publications:
i787 provides signals for C-KIT receptor internalization and functionality that control mast cell survival and development
Blood, Oct 2010; 116: 2665 - 2675.
C-KIT receptor in the human vas deferens: distinction of mast cells, interstitial cells and interepithelial cells
Reproduction, Mar 2008; 135: 377 - 384.
functional interaction of C-KIT receptor and interleukin-3 receptor.
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 2228.
expression of C-KIT receptor tyrosine kinase and effect on -cell development in the human fetal pancreas
Am J Physiol Endocrinol Metab, Aug 2007; 293: E475 - E483.
the C-KIT receptor protein in the testis of green frog rana esculenta: seasonal changes in relationship to testosterone titres and spermatogonial proliferation
Reproduction, Jan 2007; 133: 51 - 60.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human CD117 Recombinant (SCFR)
product_icon_8.jpg Mouse CD117 Recombinant (SCFR)
 
 
 
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