Optimized DNA sequence encoding extracellular domain of human SCF receptor (CD117) isoform 2 including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant C-Kit receptor (CD117) is a monomer protein consisting of 502 amino acid residue subunits, due to glycosylation migrates as an approximately 80kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant CD117 activity was measured by its binding ability in a functional ELISA. Immobilized human KIT at 20 μg/ml can bind biotinylated mouse KITL with a linear ranger 1 - 6 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human c-kit receptor is supplied as a 0.2 μm filtered PBS solution, pH7.2.
Recombinant human CD117, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The c-Kit receptor consists
of an extracellular portion
with five immunoglobulin-
like regions and an
kinase domain. The first
domains form a binding site
for its ligand, stem cell
factor (SCF), the fourth is
involved in c-Kit receptor
dimerization, and the
function of the fifth is, as
Binding of SCF induces
homodimerization of the c-
Kit receptor, followed by an
increase in receptor
and recruitment of the c-Kit
i787 provides signals for C-KIT receptor internalization and functionality that control mast cell survival and development
2665 - 2675.
C-KIT receptor in the human vas deferens: distinction of mast cells, interstitial cells and interepithelial cells
377 - 384.
functional interaction of C-KIT receptor and interleukin-3 receptor.
Blood (ASH Annual Meeting Abstracts),
expression of C-KIT receptor tyrosine kinase and effect on -cell development in the human fetal pancreas
Am J Physiol Endocrinol Metab,
E475 - E483.
the C-KIT receptor protein in the testis of green frog rana esculenta: seasonal changes in relationship to testosterone titres and spermatogonial proliferation
51 - 60.