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Human CD114 Recombinant (GCSFR)

G-CSFR

accession:  Q99062
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  :  Ig-like C2
Gene  :  GCSFR
Catalog no. :  RKQ99062

Source:
Optimized DNA sequence  encoding  extracellular domain of human CD114 including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant CD114 (GCSF receptor) is a monomer protein consisting of 608 amino acid residue subunits, due to glycosylation migrates as an approximately 90 kDa protein on SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
 
Biological Activity:

The activity of Recombinant human CD114 was measured by its ability to inhibit the G-CSF induced proliferation of NFS60 mouse myeloid cells. The ED50 for this effect is typically 20-80 ng/mL.

 

Endotoxin:

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant GCSFR (CD114) is supplied as a 0.2 μm filtered PBS solution, pH7.2.

              
Storage:
Recombinant CD114 as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

G-CSFR

The mature GCSF receptor (GCSF-R) is composed of an extracellular region, which consists of an Ig-like domain, a cytokine receptor homologous (CRH) domain, three fibronectin type III- like domains, a transmembrane region, and a cytoplasmic domain (4). The CRH domain is further divided into an N-terminal (BN) and a C-terminal (BC) domain containing a WSXWS sequence motif that is highly conserved in the CRH domain of cytokine receptors.

Related Publications:
lrg-accelerated differentiation defines unique G-CSFR signaling pathways downstream of pu.1 and c/ebp that modulate neutrophil activation
J. Leukoc. Biol., May 2008; 83: 1277 - 1285.
characterization of a juxtamembrane lysine as a determinant in lysosomal routing and signal downregulation of the activated g-csf receptor (G-CSFR).
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 2190.
granulocyte colony stimulating factor (g-csf) and G-CSFR autocrine loop promotes invasion of bladder cancer cells
AACR Meeting Abstracts, Apr 2005; 2005: 953.
structural determinants for signal transducer and activator of transcription (stat) 3 recruitment and activation by the granulocyte colony-stimulating factor receptor (G-CSFR) at phosphotyrosine ligands 704 and 744.
Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 2169.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human CD114 Recombinant (GCSFR)
 
 
 
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