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Human HIV-1 gp120 Recombinant

HIV-1 gp120

accession: 
   Size A     0.1mg   $ 400
   Size B      0.5mg  $2100
   Size C      1mg  $ 3200
Domain  : 
Gene  :  gp120
Catalog no. :  RKP045781
Source:
DNA sequence encoding fulllength of HIV-1 gp120 fused to a C terminal His tag was expressed in HEk293 cells .

 
Molecular weight:

Recombinant HIV-1 Glycoprotein 120 contains 486 amino acids and has a calculated mass of  55kDa , due to glycosylation migrates as an approximately 100 kDa protein under  reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE.
 
Activity:

Measured by its ability to bind human CD4 in functional ELISA.

 

 

 
Presentation:
Lyophilized from PBS pH-7.4, 5% trehalose.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

HIV-1 gp120

gp120 is a glycoprotein exposed on the surface of the HIV envelope.The glycoprotein gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. It is involved in entry into cells by binding to CD4 receptors, particularly helper T-cells. ince CD4 receptor binding is the most obvious step in HIV infection, gp120 was among the first targets of HIV vaccine research. Efforts to develop HIV vaccines targeting gp120, however, have been hampered by the chemical and structural properties of gp120, which make it difficult for antibodies to bind to it. gp120 can also easily be shed from the surface of the virus and captured by T cells due to its loose binding with gp41. A conserved region in the gp120 glycoprotein that is involved in the metastable attachment of gp120 to CD4 has now been identified and targeting of invariant region has been achieved with a broadly neutralising antibody,

Related Publications:
structure-function analysis of HIV-1 gp120 amino acid mutations associated with resistance to the ccr5 co-receptor antagonist vicriviroc
J. Virol., Sep 2009; 10.1128/JVI.01351-09.
HIV-1 gp120-induced migration of dendritic cells is regulated by a novel kinase cascade involving pyk2, p38 map kinase and lsp1
Blood, Aug 2009; 10.1182/blood-2009-02-206342.
x4 HIV-1 gp120 down-modulates expression and immunogenicity of co-delivered antigens
J. Virol., Aug 2009; 10.1128/JVI.00394-09.
llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (hiv-1)-neutralizing properties and high affinity for HIV-1 gp120
J. Virol., Dec 2008; 82: 12069 - 12081.
cannabinoids inhibit HIV-1 gp120-mediated insults in brain microvascular endothelial cells
J. Immunol., Nov 2008; 181: 6406 - 6416.




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Antibodies:
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product_icon_8.jpg Human HIV-1 gp120 Recombinant
product_icon_8.jpg Human HIV-1 gp120 Recombinant
 
 
 
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