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Human TGF Beta-1 Recombinant

TGF beta

accession:  P01137
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      500ug  $ 3500
Domain  :  cystine knot growth factor
Gene  :  TGFB1
Catalog no. :  RKP011371

Source:
Optimized DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells

 
Molecular weight:

Native human TGF-b1, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 26 kDa.

Recombinant TGF-beta 1 is a disulfide-linked homodimer  protein consisting of 2x113 amino acid residue subunits. TGF-beta 1 migrates as an approximately  12 - 13 kDa protein under reducing conditions and as 26kDa under nonreducing conditions .


 
Purity:
>95%, as determined by SDS-PAGE and HPLC.

 
Biological activity:

 

The ED(50) was determined by the ability to inhibit the proliferation of mink lung epithelial  cells 0.05-0.1  ng/ml, corresponding to a specific activity of 2x 107 units/mg.

 

Protein Sequence:
        10         20         30         40         50         60 
MPPSGLRLLL LLLPLLWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR GQILSKLRLA 

        70         80         90        100        110        120 
SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESAEPEPEPE ADYYAKEVTR VLMVETHNEI 

       130        140        150        160        170        180 
YDKFKQSTHS IYMFFNTSEL REAVPEPVLL SRAELRLLRL KLKVEQHVEL YQKYSNNSWR

       190        200        210        220        230        240 
YLSNRLLAPS DSPEWLSFDV TGVVRQWLSR GGEIEGFRLS AHCSCDSRDN TLQVDINGFT

       250        260        270        280        290        300 
TGRRGDLATI HGMNRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI 

       310        320        330        340        350        360 
DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA 

       370        380        390 
LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant TGF-b1 is lyophilized from  0.2 μm filtered PBS solution pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months.


Avoid repeated freeze/thaw cycles.


 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

TGF-beta 1

TGF-beta is the prototype of a protein family known as the TGF-beta superfamily. This family includes Inhibins, Activin A, MIS (Müllerian inhibiting substance), BMP (bone morphogenetic proteins), dpp (decapentaplegic) and Vg-1. Type 1 receptors and type 2 receptors have a molecular mass of 53 and 75 kDa, respectively. Signaling through the receptor requires heterodimeric complexes between both types of receptors. The type 2 receptor has been shown to bind the ligand, but it is incapable of mediating TGF- beta responses in the absence of the type 1 receptor. The type 1 receptor is expressed predominantly in hematopoietic progenitor cells. The type 2 receptor encodes a protein with an intracellular domain that functions as a serine/threonine kinase. TGF-beta is the most potent known growth inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neuronal cells, lymphoid cells and other hematopoietic cell types. TGF-beta inhibits the proliferation of T- lymphocytes by down- regulating predominantly IL- 2 mediated proliferative signals. It also inhibits the growth of natural killer cells in vivo and deactivates macrophages. TGF-beta blocks the antitumor activity mediated in vivo by IL-2 and transferred lymphokine- activated or tumor infiltrating lymphocytes.

Related Publications:
cell cycle arrest by tgf-1 enhances rsv replication in lung epithelial cells
J. Virol., Sep 2009; 10.1128/JVI.00806-09.
cutting edge: tgf-β1 and il-15 induce foxp3+ regulatory t cells in the presence of antigen stimulation
J. Immunol., Sep 2009; 183: 3574 - 3577.
nuclear factor i-c links pdgf and tgf-1 signaling to skin wound healing progression
Mol. Cell. Biol., Sep 2009; 10.1128/MCB.01921-08.
mesenchymal stem cells produce wnt isoforms and tgf1 that mediate proliferation and pro-collagen expression by lung fibroblasts
Am J Physiol Lung Cell Mol Physiol, Sep 2009; 10.1152/ajplung.90347.2008.
mechanical forces and tgfβ1 reduce podocyte adhesion through 3β1 integrin downregulation
Nephrol. Dial. Transplant., Sep 2009; 24: 2645 - 2655.




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