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Mouse IL-10 receptor beta (IL10RB) Recombinant

IL-10RB

accession:  Q61190
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  :  Extracellular
Gene  :  IL10RB
Catalog no. :  RKQ61190
Source:
DNA sequence encoding extracellular domain of Mouse IL-10RB fused to a C-terminal polyHis tag was expressed in HEK cells.

 
Molecular weight:

Recombinant Mouse Interleukin-10RB  is a monomer consisting of 212 amino acid residue subunits, and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>90%, as determined by SDS-PAGE and HPLC

 
 
Protein Sequence:

       10         20         30         40         50         60 
MAPCVAGWLG GFLLVPALGM IPPPEKVRMN SVNFKNILQW EVPAFPKTNL TFTAQYESYR 

        70         80         90        100        110        120 
SFQDHCKRTA STQCDFSHLS KYGDYTVRVR AELADEHSEW VNVTFCPVED TIIGPPEMQI 

       130        140        150        160        170        180 
ESLAESLHLR FSAPQIENEP ETWTLKNIYD SWAYRVQYWK NGTNEKFQVV SPYDSEVLRN 

       190        200        210        220        230        240 
LEPWTTYCIQ VQGFLLDQNR TGEWSEPICE RTGNDEITPS WIVAIILIVS VLVVFLFLLG 

       250        260        270        280        290        300 
CFVVLWLIYK KTKHTFRSGT SLPQHLKEFL GHPHHSTFLL FSFPPPEEAE VFDKLSIISE 

       310        320        330        340 
ESEGSKQSPE DNCASEPPSD PGPRELESKD EAPSPPHDDP KLLTSTSEV 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant IL10RB was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-10 receptor

IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73 % amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of Th1 and Th2 cells.

Related Publications:
genetic variation in the il-10 pathway modulates severity of acute graft-versus-host disease following hematopoietic cell transplantation: synergism between il-10 genotype of patient and IL-10 receptor genotype of donor
Blood, Dec 2005; 106: 3995 - 4001.
association of il-10 and IL-10 receptor gene polymorphisms and graft-versus-host disease following hematopoietic cell transplantation.
Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 421.
the expanded family of class ii cytokines that share the IL-10 receptor-2 (il-10r2) chain
J. Leukoc. Biol., Aug 2004; 76: 314 - 321.
cutting edge: il-26 signals through a novel receptor complex composed of il-20 receptor 1 and IL-10 receptor 2
J. Immunol., Feb 2004; 172: 2006 - 2010.
inhibition of IL-10 receptor function in alveolar macrophages by toll-like receptor agonists
J. Immunol., Feb 2004; 172: 2613 - 2620.




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product_icon_8.jpg Human IL-10 receptor beta (IL10RB) Recombinant
product_icon_8.jpg Mouse IL-10 receptor beta (IL10RB) Recombinant
 
 
 
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