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Rat Interleukin-13 Recombinant

IL-13

accession:  P42203
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  4 helix cytokine core
Gene  :  IL13
Catalog no. :  RKP42203
Source:
Optimized DNA sequence encoding rat Interleukin-13 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Rat IL-13, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 12 kDa.

Recombinant Rat IL-13 is a disulfide-linked monomer protein consisting of 111 amino acid residue subunits,and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of TF-1 cells was ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MALWVTAVLA LACLGGLATP GPVRRSTSPP VALRELIEEL SNITQDQKTS LCNSSMVWSV 

        70         80         90        100        110        120 
DLTAGGFCAA LESLTNISSC NAIHRTQRIL NGLCNQKASD VASSPPDTKI EVAQFISKLL 

       130 
NYSKQLFRYG H 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse IL-13  was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-13

Mouse IL13 is produced primarily by activated Th2 cells. Human IL13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL13 receptor has weak binding activity for IL13. High affinity receptors are formed when the receptor alpha chains of IL13 and IL4 receptors are coexpressed. The receptors for IL13 and IL4 share a component,a common gamma subunit that is found also in the IL2 receptor.Different IL13 receptor structures have been shown to exist on various cell types and the IL13 receptor may share more than one component with IL4 receptor. IL13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL13 enhances the production of the IL1 receptor antagonist IL1ra. IL13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.

Related Publications:
interleukin-4 (il-4) and IL-13 suppress excessive neutrophil infiltration and hepatocyte damage during acute murine schistosomiasis japonica
Infect. Immun., Jan 2012; 80: 159 - 168.
IL-13 regulates th17 secretion of il-17a in an il-10–dependent manner
J. Immunol., Dec 2011; 10.4049/jimmunol.1102216.
IL-13 receptor α1 differentially regulates aeroallergen-induced lung responses
J. Immunol., Nov 2011; 187: 4873 - 4880.
potential role of IL-13 in neuroprotection and cortical excitability regulation in multiple sclerosis
Multiple Sclerosis Journal, Nov 2011; 17: 1301 - 1312.
IL-13 induces connective tissue growth factor in rat hepatic stellate cells via tgf-β–independent smad signaling
J. Immunol., Sep 2011; 187: 2814 - 2823.




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