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Rat GRO-beta (CXCL2) Recombinant

GRO-beta

accession:  P30348
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CXCL2
Catalog no. :  RKP30348
Source:
Optimized DNA sequence encoding rat GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native rat GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant GRO-beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract rat neutrophils cells using a concentration range of 10-100 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MAPPTRQLLN AVLVLLLLLA TNHQGTGVVV ASELRCQCLT TLPRVDFKNI QSLTVTPPGP 

        70         80         90        100 
HCAQTEVIAT LKDGHEVCLN PEAPLVQRIV QKILNKGKAN 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant rat GRO-beta/CXCL2 was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution inPBS, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

CXCL2

Growth regulated oncogene- gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity)and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Related Publications:
cxcl1 regulates pulmonary host defense to klebsiella infection via CXCL2, cxcl5, nf-b, and mapks
J. Immunol., Nov 2010; 185: 6214 - 6225.
identification of two novel chemokines (ccl2 and CXCL2) in b-chronic lymphocytic leukaemia (b-cll) and prolonged survival of primary b-cll cells in vitro
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3157.
toll-like receptor 4 ligation on intrinsic renal cells contributes to the induction of antibody-mediated glomerulonephritis via cxcl1 and CXCL2
J. Am. Soc. Nephrol., Jun 2007; 18: 1732 - 1739.
selective local pmn recruitment by cxcl1 or CXCL2/3 injection does not cause inflammatory pain
J. Leukoc. Biol., May 2006; 79: 1022 - 1032.
relative contribution of lix/cxcl5, kc/cxcl1, and mip–2/CXCL2 in lps keratitis
Invest. Ophthalmol. Vis. Sci., May 2006; 47: 4361.




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