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Mouse GRO-alpha (CXCL1)Recombinant

GRO-alpha

accession:  P12850
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CXCL1
Catalog no. :  RKP12850
Source:
Optimized DNA sequence encoding mouse GRO alpha mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native mouse CXCL1/GRO-alpha is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. 

Recombinant mouse CXCL1/GRO-a is a disulfide-linked homodimeric protein consisting of 73 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

 

 

Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by the ability to chemoattract human neutrophils using a concentration range of 10.0-50.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MIPATRSLLC AALLLLATSR LATGAPIANE LRCQCLQTMA GIHLKNIQSL KVLPSGPHCT 

        70         80         90 
QTEVIATLKN GREACLDPEA PLVQKIVQKM LKGVPK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse GRO-alpha/CXCL1 was lyophilized from a 0.2μm filtered PBS pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

GRO-alpha

Growth regulated oncogene- alpha belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity)and the new designation is CXCL1. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Related Publications:
a potential role of the cxc chemokine gro in atherosclerosis and plaque destabilization: downregulatory effects of statins
Arterioscler Thromb Vasc Biol, May 2008; 28: 1005 - 1011.
hsv infection and hsv-immune complex both trigger production of neutrophil activating molecules (il-8,gro- and gm-csf) in ocular mucosal cells
Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 492.
mice deficient in the cxcr2 ligand, cxcl1 (kc/gro-), exhibit increased susceptibility to dextran sodium sulfate (dss)-induced colitis
Innate Immunity, Apr 2008; 14: 117 - 124.
gro is highly expressed in adenocarcinoma of the colon and down-regulates fibulin-1
Clin. Cancer Res., Oct 2006; 12: 5951 - 5959.
gro- regulation in airway smooth muscle by il-1 and tnf-: role of nf-b and map kinases
Am J Physiol Lung Cell Mol Physiol, Jul 2006; 291: L66 - L74.




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