Description
Accession
O88430
Source
Optimized DNA sequence encoding Mouse MDC (CCL22) mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse MDC/CCL22 has a calculated molecular mass of approximately8 kDa. Recombinant MDC is a disulfide-linked homodimeric protein consisting of 67 amino acid residue subunits, andmigrates as an approximately8 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Biological Activity
Determined by its ability to chemoattract human T cells using a concentration range of 10.0-50.0 ng/ml.
Presentation
Recombinant mouse CCL22 is presented as 0.2μm filtered PBS pH 7.5 .
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Methods
FACS analysis
- HLA-A2 molecules are stained on the surface of DC with BB7.2 monoclonal antibody (HB82, ATCC) for 45 min on ice followed by a secondary antibody conjugated with phycoerythrin (PE) (goat anti-mouse IgG 115-116-146) for 45 min.
- The intracellular protein expression in ivt-NA-transfected mC is detected using the following primary antibodies: tyrosinase-specific primary monoclonal antibody (clone T311; diluted 1:10 600741), melan-A-specific primary monoclonal antibody (clone A103; diluted 1:20, ) and survivin-specific primary monoclonal antibody (clone 91630; diluted 1:2.5& , ) followed by staining with Cy5-conjugated secondary antibody (rat anti-mouse IgG Immunoesearch, 415-176-166).
- The intracellular staining is performed as follows.
- The mDC samples are first fixed in FACS buffer containing 1% paraformaldehyde for 30 min on ice and washed twice in buffer without additives and once in buffer containing 0.1% saponin .
- Samples are then incubated with primary antibody in buffer containing 0.25% saponin for 1 h at room temperature, washed once…