Description
Accession
O00626
Source
Optimized DNA sequence encoding Human MDC (CCL22) mature chain was expressed in Escherichia Coli.
Molecular weight
Native human MDC/CCL22 has a calculated molecular mass of approximately 8 kDa. Recombinant MDC is a monomer protein consisting of 69 amino acid residue subunits, and migrates as an approximately 8 kDa protein under reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Protein Sequence
MDRLQTALLV VLVLLAVALQ ATEAGPYGAN MEDSVCCRDY VRYRLPLRVV KHFYWTSDSC PRPGVVLLTF RDKEICADPR VPWVKMILNK LSQ
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Biological Activity
Determined by its ability to chemoattract human T cells using a concentration range of.0-50.0 ng/ml.
Presentation
Recombinant CCL22 is presented as.2μm filtered PBS pH.5 .
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P51679
Biological Process
Molecular function
Methods
Chemokine transcripts induced in human islet cells in response to inflammatory stimuli (microarray analysis).
- Cytokine cocktail -induced expression by a factor of >30 was observed for CCL5, CCL8, CCL22, CX3CL1, CXCL9, and CXCL10 (asterisks indicate significant differences between control and cytokine-treated islets).
