Description
Accession
Q9UHF1
Source
DNA sequence encoding mature chain of Human EGFL7(VE-Statin) to a C-terminal polyHis tag was expressed in Insect cells.
Molecular weight
Recombinant Human EGFL7 is a monomer consisting of 268 amino acid residue , and migrates as an approximately31 kDa protein under reducing conditions in SDS-PAGE.
Purity
>90%, as determined by SDS-PAGE
Biological Activity
The activity was determined by the ability to bind human NOTCH1 in functional ELISA.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGFL7 is lyophilized from 0.2 μm filtered 100mM glycine, 10mM NaCl, 10% Glycerol solution pH 7.0 and 5% trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.. It is recommended to add 0.1% BSA for long term storage of reconstituted product.
Storage
Lyophilized EGFL7 , as supplied, should be storedat -20°C to-70°C Upon reconstitution , working aliquots may be stored at 2° - 8° C for one month, or six months at -20C without detectable loss of activity. It is recommended to add 0.1% BSA for long term storage. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Biological Process
Molecular function