/, Cytokines/Human IL-28B Recombinant

Human IL-28B Recombinant

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$160.00$3,500.00

SKU: RKQ8IZI9 Tags: , , , , , , , , , , ,

Description

Accession
Q8IZI9
Source
DNA sequence encoding mature chain of Human IL28B to a C-terminal polyHis tag was expressed in CHO cells.
Molecular weight
Recombinant Human Interleukin-28B (Interferon lambda) is a monomer consisting of 186 amino acid residue , and migrates as an approximately23 kDa protein under reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE
Biological Activity
The activity was determined by a WISH cell viral resistance assay with a linear range of 4-20 ng/ml .
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Interleukin-28B was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function

Methods

pDC-GEN2.2 cells sense HCV PAMP and produce Type I and Type III IFNs.

  • H) Western Blot of pDC-GEN2.2 cell lysates for IL-28B/IFNλ3 after 24 hours of HCV PAMP stimulation.
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