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Human IL-12 receptor beta 1 Recombinant

IL-12

accession:  P42701
   Size A     5ug   $ 90
   Size B      25ug  $190
   Size C      500ug  $ 1950
Domain  :  Extracellular
Gene  :  IL12RB1
Catalog no. :  RKP42701
Source:
DNA sequence encoding  extracellular domain of Human IL-12RB1 fused to a C-terminal polyHis tag was expressed in HEK cells.

 
Molecular weight:

Recombinant Human IL12RB1  is a monomer consisting of 528 amino acid residue subunits, and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was determined by its ability to bind Human IL-12B in a linear range of 2-60 ng/ml in functional ELISA .

 
Protein Sequence:

 

        10         20         30         40         50         60 
MEPLVTWVVP LLFLFLLSRQ GAACRTSECC FQDPPYPDAD SGSASGPRDL RCYRISSDRY 

        70         80         90        100        110        120 
ECSWQYEGPT AGVSHFLRCC LSSGRCCYFA AGSATRLQFS DQAGVSVLYT VTLWVESWAR 

       130        140        150        160        170        180 
NQTEKSPEVT LQLYNSVKYE PPLGDIKVSK LAGQLRMEWE TPDNQVGAEV QFRHRTPSSP 

       190        200        210        220        230        240 
WKLGDCGPQD DDTESCLCPL EMNVAQEFQL RRRQLGSQGS SWSKWSSPVC VPPENPPQPQ 

       250        260        270        280        290        300 
VRFSVEQLGQ DGRRRLTLKE QPTQLELPEG CQGLAPGTEV TYRLQLHMLS CPCKAKATRT 

       310        320        330        340        350        360 
LHLGKMPYLS GAAYNVAVIS SNQFGPGLNQ TWHIPADTHT EPVALNISVG TNGTTMYWPA 

       370        380        390        400        410        420 
RAQSMTYCIE WQPVGQDGGL ATCSLTAPQD PDPAGMATYS WSRESGAMGQ EKCYYITIFA 

       430        440        450        460        470        480 
SAHPEKLTLW STVLSTYHFG GNASAAGTPH HVSVKNHSLD SVSVDWAPSL LSTCPGVLKE 

       490        500        510        520        530        540 
YVVRCRDEDS KQVSEHPVQP TETQVTLSGL RAGVAYTVQV RADTAWLRGV WSQPQRFSIE 

       550        560        570        580        590        600 
VQVSDWLIFF ASLGSFLSIL LVGVLGYLGL NRAARHLCPP LPTPCASSAI EFPGGKETWQ 

       610        620        630        640        650        660 
WINPVDFQEE ASLQEALVVE MSWDKGERTE PLEKTELPEG APELALDTEL SLEDGDRCKA 


KM 

 

(*)Complete precursor sequence shown, expressed chain highlighted 

 

Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Interleukin-12 receptor beta was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-12 receptor beta

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Defects in IL12RB1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X- linked inheritance

Related Publications:
il-11 receptor in the pathogenesis of il-13-induced inflammation and remodeling
J. Immunol., Feb 2005; 174: 2305 - 2313.
recombinant soluble interleukin-11 (il-11) receptor -chain can act as an il-11 antagonist
Blood, Dec 1997; 90: 4403 - 4412.




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