Mouse IL-12 Recombinant

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$160.00$2,900.00

SKU: RKP43431 Tags: , , , ,

Description

Accession
P43431
Source
DNA sequence encoding extracellular domain of mouse IL-12A fused to a C-terminal polyHis tag and aDNA sequence encoding extracellular domain of mouse IL-12Bfused to a C-terminal polyHis tagwere co-expressed in HEK cells.
Molecular weight
Recombinant mouse Interleukin-12 is a heterodimer consisting of amino acid residue subunits (203 subunit A + subunit B), and migrates as an approximately46 kDa protein under reducing conditions in SDS-PAGE.
Purity
>90%, as determined by SDS-PAGE
Biological Activity
The activity was determined by its ability to bind recombinant Mouse IL-12RB in functional ELISA .
Protein Sequence
MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Interleukin-12 was lyophilized from a.2 μm filtered PBS solution pH7.4 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O35228
Molecular function
Molecular function
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