Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml.
Immunohistochemical analysis of BDNF expression in paraffin embedded formalin fixed Rat brain tissue.
Cat# RPA23560 was used as primary antibody, dilution 1:200.
Polyclonal BDNF antibody was produced from sera of rabbits immunized with synthetic protein containing BDNF antigenic determinants.
Serum IgG fraction was purified by Protein A affinity chromatography.
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
endogenous BDNF regulates inhibitory synaptic transmission in the ventromedial nucleus of the hypothalamus.
postsynaptic trpc1 function contributes to BDNF-induced synaptic potentiation at the developing neuromuscular junction
14754 - 14762.
levels of BDNF impact oligodendrocyte lineage cells following a cuprizone lesion
14182 - 14190.
spatial segregation of BDNF transcripts enables BDNF to differentially shape distinct dendritic compartments
16813 - 16818.
brain-derived neurotrophic factor (BDNF) enhances gaba transport by modulating the trafficking of gaba transporter-1 (gat-1) from the plasma membrane of rat cortical astrocytes
J. Biol. Chem.,