Human Interleukin-22 Recombinant (E.Coli)
Category: Interferon-IL10 family$70.00 – $4,700.00
Description
Accession
Q9GZX6
Source
Optimized DNA sequence encoding Human Interleukin-22 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human IL-22 has a calculated molecular mass of approximately 17 kDa. Recombinant IL-22 is a disulfide-linked homodimer protein consisting of 2x147 amino acid residue subunits, andm igrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Activitywas determined by the ability to inhibit growth of human A549 cancer cells at concentrations ranging between-100 ng/ml.
Protein Sequence
MAALQKSVSS FLMGTLATSC LLLLALLVQG GAAAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-22 was lyophilized from.2μm filtered concentrated (1.0mg/ml) solution inmM NaCl,mM Tris, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Q969J5-2
Interactor
Q969J5-3
Molecular function
Methods
IL-22 enhances miR-197 expression.
- IL-22 was added to PHK cells at the indicated concentrations.
The neoplastic area of CRC samples is massively infiltrated with Th17-related cytokine-, TNF-α- and IL-6-producing cells.
- (a) IFN-γ, IL-17A, IL-17F, IL-21, IL-22, TNF-α and IL-6 proteins were analyzed by enzyme-linked immunosorbent assay in LPMC-derived supernatants (LPMC SNs) and TIL-derived supernatants (TL SNs), and data are expressed as pg/ml supernatants.
