Mouse Interleukin-25 Recombinant

Mouse Interleukin-25 Recombinant
IL-25

accession:

Q9CPT4

Size A	2ug	$70
Size B	10ug	$160
Size C	1mg	$ 4700

Domain :

4 Helix Cytokine

Gene :

IL25

Catalog no. :

RKQ9CPT4

Source:

Optimized DNA sequence encoding Mouse Interleukin-25 mature chain was expressed in Escherichia Coli.

Molecular weight:

Native Mouse IL-25 has a calculated molecular mass of approximately 16 kDa.

Recombinant IL-25 is a disulfide-linked monomeric protein consisting of 143 amino acid residue subunits, and migrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE.

Purity:

>95%, as determined by SDS-PAGE and HPLC

Biological Activity:

Activity was determined by the ability to induce IL-8 production in human PBMCs at concentrations ranging between 10-50 ng/ml.

Protein Sequence:

        10         20         30         40         50         60 
MAAPSGGFWT AVVLAAAALK LAAAVSEPTT VPFDVRPGGV VHSFSQDVGP GNKFTCTFTY 

        70         80         90        100        110        120 
ASQGGTNEQW QMSLGTSEDS QHFTCTIWRP QGKSYLYFTQ FKAELRGAEI EYAMAYSKAA 

       130        140        150        160 
FERESDVPLK SEEFEVTKTA VSHRPGAFKA ELSKLVIVAK AARSEL

(*)Complete precursor sequence shown, expressed chain highlighted

Endotoxin:

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Presentation:

Recombinant mouse Interleukin-25 was lyophilized from 0.2μm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.0.

Reconstitution:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Storage:

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

Usage:

This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IL-25

IL-25 (also known as IL- 17E), a member of the IL-17 family, has been implicated in Th2 cell-mediated immunity. Recently, IL-25 has been identified as one of the initiators of the Th2 response (10, 11) and has also been shown to be expressed by mast cells. The transgenic overexpression of IL-25 by lung epithelial cells results in mucus hyperproduction and airway infiltration of macrophages and eosinophils; conversely, IL-25 blockade reduced airway inflammation and Th2 cytokine production in an allergen-induced asthma model.

Related Publications:

	t-helper cell type 2 (th2) memory t cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma PNAS, Jan 2011; 10.1073/pnas.1014241108.
	critical role of IL-25 in nematode infection-induced alterations in intestinal function J. Immunol., Dec 2010; 185: 6921 - 6929.
	nk cell deficiency predisposes to viral-induced th2-type allergic inflammation via epithelial-derived IL-25 J. Immunol., Oct 2010; 185: 4681 - 4690.
	overexpression of smad2 drives house dust mite–mediated airway remodeling and airway hyperresponsiveness via activin and IL-25 Am. J. Respir. Crit. Care Med., Jul 2010; 182: 143 - 154.
	pulmonary il-17e (IL-25) production and il-17rb+ myeloid cell-derived th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease J. Immunol., Nov 2009; 183: 5705 - 5715.