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Rabbit anti APAF-1 Polyclonal


accession:  O14727
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA14727
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
APAF1 Immunohistochemistry/IHC

Immunohistochemical analysis of human thymus sections showing lymphocyte expression of APAF-1.

Cat# RPA14727 was used as primary antibody, dilution 1:500, DAB staining.




Polyclonal anti Apoptotic Protease Activating Factor 1 (APAF-1)  was produced from sera of rabbits  immunized with synthetic protein containing APAF-1 antigenic determinants. 
Species reactivity:
Human, Mouse, Rat, Rabbit

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Related Publications:
a new model for the transition of apaf-1 from inactive monomer to caspase-activating apoptosome
J. Biol. Chem., Nov 2009; 284: 32717 - 32724.
parcs is a dual regulator of cell proliferation and apaf-1 function
J. Biol. Chem., Sep 2008; 283: 24400 - 24405.
epigenetic dysregulation of the dap kinase/p14/hdm2/p53/apaf-1 apoptosis pathway in acute leukaemias
J. Clin. Pathol., Jul 2008; 61: 844 - 847.
developing postmitotic mammalian neurons in vivo lacking apaf-1 undergo programmed cell death by a caspase-independent, nonapoptotic pathway involving autophagy
J. Neurosci., Feb 2008; 28: 1490 - 1497.
differential apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues
PNAS, Dec 2007; 104: 20820 - 20825.

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Growth factors
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Purified Polyclonal
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