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Rabbit anti Caspase-3 Polyclonal


accession:  P42574
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA42574
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
AIF Immunohistochemistry/IHC

Immunohistochemical analysis of FFPE human colon sections showing expression of Caspase-3.

Cat# RPA42574 was used as primary antibody, dilution 1:500, DAB staining.




TYH Immunohistochemistry/IHC

Immunohistochemical analysis of FFPE NZ rabbit myocardium sections showing expression of Caspase-3. 

Cat# RPA42574 was used as primary antibody, dilution 1:200, DAB staining.




Polyclonal anti Caspase-3 (CPP32) antibody was produced from sera of rabbits  immunized with synthetic protein containing Caspase-3 antigenic determinants.
Species reactivity:
Human, Mouse, Rat, Rabbit

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Caspase 3

Related Publications:
identification of functional regions defining different activity between caspase-3 and caspase-7 within cells
J. Biol. Chem., Jun 2010; 10.1074/jbc.M110.126573.
granzyme b-induced and Caspase 3-dependent cleavage of gelsolin by mouse cytotoxic t cells modifies cytoskeleton dynamics
J. Biol. Chem., Jun 2010; 285: 18918 - 18927.
a novel plant homeodomain finger 10–mediated antiapoptotic mechanism involving repression of caspase-3 in gastric cancer cells
Mol. Cancer Ther., Jun 2010; 9: 1764 - 1774.
hlj1 is a novel caspase-3 substrate and its expression enhances uv-induced apoptosis in non-small cell lung carcinoma
Nucleic Acids Res., May 2010; 10.1093/nar/gkq412.
correlation of cisplatin response and caspase-3 cleavage in human carcinomas.
ASCO Meeting Abstracts, May 2010; 28: e21104.

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